深视3D光片显微镜

DeepSight 3D Lightsheet Microscope

深观至极,细微必见

☑ 跨尺度  ☑ 组织更大   ☑ 看得更清   ☑ 成像更快   ☑ 3D成像  ☑活体可用 

面向透明化跨尺度样本、多色三维成像与动态观察的高分辨光片平台

yh533388银河深视3D光片显微镜系列，拥有自主研发的平铺虚拟扫描光片技术，在大视野、高分辨、低光毒和三维成像效率之间取得更好的平衡，兼顾微观类器官、宏观整鼠，精准体现尺寸层级跨越。

yh533388银河深视3D光片显微镜系列可对光片状态进行实时调节和自动校准，保证其在不同应用中的易用性和稳定性。此外，根据不同样品的特性，深视3D光片显微镜系列还分别提供了优化配件以及流程方案，方便使用者调整成像分辨率、速度、放大倍数等参数以及样本的处理方法，以获得微米级、亚微米级甚至百纳米级三维空间分辨率的多色组织结构信息。配套的图像处理和分析平台让使用者可更高效地对结果进行分析和解读，广泛应用于神经、肿瘤、发育等基础生物科研领域以及药物开发、药效检测、临床病理检测等医药领域。

从微观到宏观的跨尺度成像 

优秀的光学设计配合多规格成像仓，使光束能在数厘米的距离内灵活调整聚焦——无论是数百微米的类器官、毫米级的组织，还是厘米级的整只小鼠，都能获得精准、清晰的成像。超长位移行程与超高步进精度相辅相成，在提供充足成像长度的同时，保留了纳米级别的定位能力。搭配长工作距离、高数值孔径的物镜，既保证了大视野下的成像宽度，又不牺牲分辨率。系统支持完整扫描一只成年小鼠，而针对类器官等微小样本，也可切换至高倍，轻松捕捉内部结构细节。真正实现一机多能，让跨尺度研究变得简单高效。

全品类透明化样本一站式3D成像 

透明化样本的成像方案，往往取决于组织类型、荧光标记方式、样本本身大小以及所采用的透明化体系。深视3D光片显微镜系列面向这类复杂样本设计，可匹配亲水型、疏水型和水凝胶型等各类透明化方法。对于透明化鼠脑、心脏、肝脾肺肾、胚胎、脊髓等样本，完整三维成像的价值在于保留结构的连续性，研究者不必只依赖局部切片或多次分割后的拼接结果，而可以在同一个体数据中观察组织整体形态、空间分布和局部细节。

自研平铺光片技术，画质与成像稳定性双提升

深视3D光片显微镜系列可选择由yh533388银河自主研发的平铺虚拟扫描光片技术，在大视野内对光片状态进行调控和优化。相比固定光片一次性覆盖整个视野，平铺方式更利于在较大观察范围内维持光片厚度、照明均匀性和有效层切能力，从而缓解视野、分辨率与信噪比之间的矛盾。

光束自动校准易用性与可靠性兼顾

在不同折射率成像液、不同激发波长和不同放大倍率下，光片位置可能出现横向或 Z 向偏移。深视3D光片显微镜系列支持光片校准，可帮助用户在多色成像和不同透明化体系中保持更好的空间一致性，减少反复手动调光带来的不确定性。配备快扫模式可用于样本预览和目标区域定位，高分辨模式则用于关键结构验证，使实验流程从筛选到精细采集更连贯。

成年小鼠皮肤（4色）

采用anti-Vimentin(绿色）、anti-CD34（黄色）、anti-CK-15（洋红色）、DAPI（蓝色）共同标记

低毒高速成像，适配活体动态长时观测    

将照明与检测分离，只激发当前成像层面的荧光信号。配合面阵相机并行采集，能够在较低无效激发的条件下获取三维图像，有助于降低光漂白与光毒性，配合自主研发的活体模块适合对斑马鱼、类器官等样本进行活体或长时程观察。

St.23活体海鞘胚胎

面向大数据的完整工作流

对于用户而言，深视 3D 光片显微镜系列不只是一台显微镜主机，也是一套从采集控制、实时预览、数据预处理到自动拼接的成像工作流入口。系统控制软件完成指定区域的数据采集后，可灵活调用配套图像处理和分析平台完成后续处理、观察、分析工作，帮助用户把大样本图像转化为可观察、可比较、可分析的三维结果。

应用方向

2026（11篇）

Cell Stem Cell

Psychological stress drives aging-like hematopoietic stem cell dysfunction through a brain-gut-bone marrow axis

Neuron

Piriform cortex gates learned fear through a brain spleen neuroimmune axis

Cell Stem Cell

Human retinal organoids functionally bridge a transected optic nerve in a rat modelHuman retinal organoids functionally bridge a transected optic nerve in a rat model

SSRN（预印）

A Biphasic Hydrogel Prevents Glioblastoma Recurrence by Activating an EZH2-NeuroD1-SRCIN1 Neuronal Conversion Axis

Diagnostics

A Novel Rapid 3D Tissue-Clearing and Staining Approach for Enteric Neurovascular Imaging and Pathology Applications

Nature Cell Biology

Perivascular spread of CSF-derived α-synuclein oligomers drives region-specific Parkinson's-like pathology

Cell Reports Medicine

Functional human salivary gland organoids for tissue regeneration in chemically defined culture systems

Cell Reports

Ccl12 coordinates immune-neural crosstalk to promote adipose sympathetic remodeling after burn trauma

Nature

Fibre integrated circuits by a multilayeredspiral architecture

Cell Reports

Ccl12 coordinates immune-neural crosstalk to promote adipose sympathetic remodeling after burn trauma

eLife

Symmetric brain-liver circuits mediate lateralized regulation of hepatic glucose output

2025（21篇）

Molecular Therapy

Niche environment remodeling promotes long-term endometrium stromal stem cell engraftment and tissue regeneration in thin endometrium

Journal of Ethnopharmacology

Baicalin ameliorates ulcerative colitis by regulating macrophage polarization through the JAK1/STAT1/SOCS1 pathway

Optics EXPRESS

DLLP: a deep learning-based layer prediction network for three-dimensional fluorescence microscopy

Nature Communications

Migration of CD8+ TSCM cells into intestine via PPBP-CXC axis increases host stress susceptibility by inhibiting gut microbiome-derived homovanillic acid

Cell Metabolism

Galnt2 neurons in the ventromedialhypothalamus counterregulatehypoglycemia via a brain-liver neurocircuit

Signal Transduction and Targeted Therapy

Rapid amyloid-β clearance and cognitive recovery through multivalent modulation of blood–brain barrier transport

Advanced Science

Mesenchymal Stromal Cells Play an Analgesic Role Through a Npy2r Sensory Neuron-Mediated Lung-to-Brain Axis

Brain, Behavior, and Immunity

DCX+ cells cripple systemic spleen immunity and promote tumor progression

EMBO Molecular Medicine

Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

Science Translational Medicine

Targeted suppression of glioma by ultralow-dose x-ray–induced photodynamic therapy and gold-based nanoclusters in preclinical models

作者：Shi Li, Shileng Chen, Yimin Li, Wenjing Sun, Junjun Li, Huaping Deng, Haizhen Ding,Zongzhang Wang, Luchao Zhu, Jun Liu, Minjie Wang, Yushuo Feng, Yuanyuan Xie,Yiru Wang, Yaqing Liu, Wenle Li, Qin Lin, Xiaobing Jiang, Hongmin Chen

Scientific Reports

Application of HEMA-AAm copolymer to achieve faster optical tissue transparency for 2D/3D fluorescence imaging

作者：Ma, Ruixiu Qin, Qiufeng Yao, Yier Li, Xingshun Cong, Wenhui Wu, Qi Zhao, Hua Ye& Kefeng Wu

eLife

3D reconstruction of neuronal allometry and neuromuscular projections in asexual planarians using expansion tiling light sheet microscopy

作者：Jing Lu, Hao Xu, Dongyue Wang, Yanlu Chen, Takeshi Inoue,Liang Gao, Kai Lei

Cell Regeneration

Morphological segmentation with tiling light sheet microscopy to quantitatively analyze the three‑dimensional structures of spinal motoneurons

作者：Huijie Hu , Dongyue Wang, Yanlu Chen and Liang Gao

Nature Biomedical Engineering

Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes

作者：Jianfeng Dai1, Weijun Wei , Chenxu Yan, Ding-Kun Ji, Caiqi Liu,Jialiang Huang, Chenyi Liang, Jianjun Liu, Zhiqian Guo&Wei-Hong Zhu

Nature Photonics

Curved light sheet microscopy for centimetre-scale cleared tissue imaging

作者：Lijuan Tang, Jiayu Wang , Jiayi Ding, Junyou Sun, Xing-jun Chen,Quqing Shen, Ruiheng Song, Peng Cao , Rong Gong, Fang Xu ,Woo-ping Ge , Wenzhi Sun , Hu Zhao & Jianglai Wu

Frontiers in Aging Neuroscience

Whole-brain 3D imaging of dopaminergic neurons and glial cells in the mouse model of Parkinson’s disease induced by 6-OHDA

作者：Mengqi Wang, Linglong Xiao, Yifeng Shi, Yaping Wu,Xinyuejia Huang, Yang Wu, Yangyang Xu, Lin Bai, Wei Pan,

Jie Zhang and Wei Wang

Cell Reports

Halting hepatocellular carcinoma: Identifying intercellular crosstalk in HBV-driven disease

作者：Lingyun Zhou, Chang-Hai Liu,Duoduo Lv, Binru Cai, Yiguo Hu,Manuel Romero-Go´ mez

Journal of Neuroimmunology

3D imaging and pathological analysis of microglia in LPS-treated mice with light-sheet fluorescence microscopy

作者：Renjie Liu, Jianbo Xiu

Nature Communications

Mitochondria transplantation transiently rescues cerebellar neurodegeneration improving mitochondrial function and reducing mitophagy in mice

作者：Shu-Jiao Li, Qian-Wen Zheng, Jie Zheng, Jin-Bao Zhang, Hui Liu,Jing-Jing Tie, Kun-Long Zhang, Fei-Fei Wu, Xiao-Dong Li, Shuai Zhang,Xin Sun, Yan-Ling Yang& Ya-YunWang

Nature Communications

Noradrenergic inputs from the locus coeruleus to anterior piriform cortex and theolfactory bulb modulate olfactory outputs

作者：Chi Geng, Ruochen Li, Shan Li,Penglai Liu, Yuxin Peng, Changyu Liu,Zhen Wang, Hongxing Zhang& Anan Li

Nature Communications

Noradrenergic inputs from the locus coeruleus to anterior piriform cortex and theolfactory bulb modulate olfactory outputs

2024（15篇）

Nature Communications

Charge-assisted stabilization of lipid nanoparticles enables inhaled mRNA delivery for mucosal vaccination

作者：Shuai Liu, Yixing Wen, Xinzhu Shan, Xinghuan Ma, Chen Yang, Xingdi Cheng, Yuanyuan Zhao, Jingjiao Li, Shiwei Mi, Haonan Huo,Wei Li, Ziqiong Jiang, Yijia Li, Jiaqi Lin, LeiMiao & Xueguang Lu

Signal Transduction and Targeted Therapy

EphrinB2-mediated CDK5/ISL1 pathway enhances cardiac lymphangiogenesis and alleviates ischemic injury by resolving post-MI inflammation

作者：Yingnan Bai'g, iming Chen’, Fanghao Guo?3, jinghong Zhang’, jinlin Hu", xuefei Tao", Qing Lu', Wenyi L?, Xueying Chen'Ting Gong', Nan Qiu', Yawei jin', Lifan Yang', Yu Lei, chengchao Ruan of, Qing jing*, john p Cooke , shijun wang 1og,Yunzeng Zou b.11@and Junbo Ge

Advanced Science

Whole-Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy

作者：Yezhou Wang, ZileJiang, Kai Zhang, Huimin Tang, Guimei Wang, Jinshan Gao,Guanghui He, Baoyue Liang, Li Li, Chaoyong Yang,* and Xianming Deng

Original Article

3D visualization of cellular and molecular distributions in human crystalline lenses at different age

作者：Wei Hu, Xiaoyu Tong, Lu Zhang, Jiajia Li, Kaijing Zhou, Wenhan Lu, Jiemei Shi, Wenjing Wang, Mengdi Zhang, Yicong Wang, Wendong Han, Liyipeng Chen, Jin Li, Yi Feng

链接：https://doi.0rg/10.62347/JAM06905

experimental eye research

A Novel Three-dimensional Method for Detailed Analysis of RGC Central Projections under Acute Ocular Hypertension

作者：Wenhan Lu, Yu Wang, Wei Hu , Xinyi Lin , Xiaoyu Tong , Yi Tian a,c , Yuning Chen , Yicong Wang , Yan Xiao  , Hongfang Yang , Yi Feng , Xinghuai Sun ,

advanced MATERIALS

Molecular Imaging of Ovarian Follicles and Tumors With Near-Infrared II Bioconjugates

作者：Yicong Wang, Wenhan Lu, Zi-Han Chen, Yan Xiao, Yu Wang, Wenhao Gao, Zhiming Wang, Ruihu Song, Zhao Fang, Wei Hu, Xiaoyu Tong, Kuinyu Lee, Zhenle Pei, Minzhen Xu, Fan Zhang, Hao Chen, Yi Feng

Neuron

Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson’s and Alzheimer’s disease co-pathology and behavior impairments

作者：Jie Xiang, Jingrong Tang, Fei Kang, Jiajun Ye, Yueying Cui, Zhentao Zhang, Jing Wang, Shengxi Wu, and Keqiang Ye

American Journal of Physiology-Endocrinology and Metabolism

Characterization of a novel adipose tissue located between abdominal lymph nodes and cervix/prostate in mice

作者：Lihao Hu, Gaochen Zhang, Xiaoyu Tong, Lulu Wang, Xiang Qiu, Hongbo Yang, Xinmei Liu, and Hefeng Huang

Research Square（预印本）

Astrocytic Bestrophin1 in the Anterior Cingulate Cortex Modulates the Formation and Persistence of Morphine Addiction Memory

作者：Linlin Sun, Zepeng Liu, Yifan Li, Xiaorui Lin, Yu Huo, Shengyang Guo, Xiang Fan, Xinzhi Ye, Yun Yao, Jiao Liu, Ning Wang, Sen Song, and Yijing Li

Science Advances

Netrin-1–engineered endothelial cell exosomes induce the formation of pre-regenerative niche to accelerate peripheral nerve repair

作者：Jinsheng Huang, Jiangnan Li , Senrui Li , Xiaoqi Yang, Nianci Huo, Qiang Chen, Wengang Wang, Ningning Yang , Yuanyi Wang  , and Nan Zhou

Nature Neuroscience

Somatosensory cortex and central amygdala regulate neuropathic pain-mediated peripheral immune response via vagal projections to the spleen

作者：Xia Zhu, Ji-Ye Huang, Wan-Ying Dong, Hao-Di Tang, Si Xu, Qielan Wu, Huimin Zhang, Ping-Kai Cheng, Yuxin Jin, Meng-Yu Zhu, Wan Zhao, Yu Mao, Haitao Wang, Yan Zhang, Hao Wang, Wenjuan Tao , Yanghua Tian , Li Bai & Zhi Zhang 

Science of The Total Environment

Kidney injury contributes to edema of zebrafish larvae caused by quantum dots

作者：Luanjin Wang , Cixin Liang , Naying Zheng , Chunyan Yang , Sen Yan , Xiang Wang , Zhenghong Zuo, Chengyong He

链接：https://doi.org/10.1016/j.scitotenv.2023.168420

nature communications

Alternations in inflammatory macrophageniche drive phenotypic and functionalplasticity of Kupffer cells

作者：Han-Ying Huang, Yan-Zhou Chen, Chuang Zhao, Xin-Nan Zheng, Kai Yu, Jia-Xing Yue, Huai-Qiang Ju, Yan-Xia Shi & Lin Tian

Journal of Ethnopharmacology

Investigating the pharmacological mechanism of Zhengyuan jiaonang for treating colorectal cancer via network pharmacology analysis and experimental verification

Nature Communications

Alternations in inflammatory macrophage niche drive phenotypic and functional plasticity of Kupffer cells

2023（13篇）

Research

Characteristic Features of Deep Brain Lymphatic Vessels and Their Regulation by Chronic Stress

作者：Junzhuang Chang, Bingqing Guo, Yan Gao, Wei Li, Xiaoyu Tong, Yi Feng, and Nashat Abumaria

Nature Metabolism

Thermogenic adipocyte-derived zinc promotes sympathetic innervation in male mice

作者：Junkun Jiang, Donglei Zhou, Anke Zhang, Wenjing Yu, Lei Du Huiwen Yuan, Chuan Zhang, Zelin Wang, Xuyang Jia Zhen-Ning Zhang & Bing Luant

Journal of Experimental & Clinical Cancer Research

Liver cancer stem cell dissemination and metastasis: uncovering the role of NRCAM in hepatocellular carcinoma

作者：Lingyun Zhou, Linye He, Chang‑Hai Liu, Huandi Qiu, Li Zheng, Klarke Michael Sample, Qin Wu, Jiaxin, Kunlin Xie, Javier Ampuero, Zhihui Li, Duoduo Lv, Miao Liu, Manuel Romero‑Gómez, Yiguo Hu and Hong Tang

Microbiology Spectrum

Spatiotemporal observations of host-pathogen interactions in mucosa during SARS-CoV-2 infection indicate a protective role of ILC2s

作者：Wei Hu, Lu Meng, Chao Wang, Wenhan Lu, Xiaoyu Tong, Rui Lin, Tao Xu, Liang Chen, An Cui, Xiaoqing Xu, Anni Li, Jia Tang, Hongru Gao, Zhenle Pei, Ruonan Zhang, Yicong Wang, Yu Wang,Wendong Han,Ning Jiang,Chenglong Xiong,Yi Feng, Kuinyu Lee, Mingquan Chen

EMBO Reports

Role and mechanism of PVN–sympathetic–adipose circuit in depression and insulin resistance induced by chronic stress

作者：Jing Wang, Linshan Sun, Jingjing You, Honghai Peng, Haijing Yan, Jiangong WangFengjiao Sun, Minghu Cui, Sanwang Wang, Zheng Zhang, Xueli Fan, Dunjiang Liu, Cuilan Liu, Changyun Qiu, Chao Chen, Zhicheng Xu, Jinbo Chen, Wei Li,& Bin Liu

BIOSENS BIOELECTRON

3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing

作者：Xiaoyan Lyu , Feiyun Cui Hang Zhou , Bo Cao , Xiaolan Zhang , Minghui Cai , Shulong Yang , Bangyong Sun , Gang Li ,

Human Brain

Study on the transparent dFC-Tesos microscopic imaging method for large tissues in human brain

作者：Di-Di Li, Xue Wang, Wen-Ying Qiu, Jing-Li Yang, Wei-Ping Wang, Xiao-Liang Wang

Nature Materials

Rapid fabrication of physically robust hydrogels

作者：Bingkun Bao, Qingmei Zeng, Kai Li, Jianfeng Wen, Yiqing Zhang, Yongjun Zheng, Renjie Zhou, Chutong Shi, Ting Chen, Chaonan Xiao, Baihang Chen, Tao Wang, Kang Yu, Yuan Sun, Qiuning Lin , Yong He, Shantung Tu & Linyong Zhu

Science China Life Science

Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity

作者：Yonghui Jiang, Xueying Gao, Yue Liu, Xueqi Yan, Huangcong Shi,Rusong Zhao, Zi-Jiang Chen, Fei Gao, Han Zhao & Shigang Zhao

Cell Proliferation

Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans

作者：Meng-Chao Zhu,Wei Hu,Lei Lin Qing-Wen Yang,Lu Zhang,Jia-Lin Xu,Yi-Tong Xu, Jia-Sheng Liu,Meng-Di Zhang,Xiao-Yu Tong,Kai-Yi Zhu,Ke Feng,Yi Feng, Jian-Zhong Su,Xiu-Feng Huang,Jin Li,

The American Journal of Pathology

Defective Uterine Spiral Artery Remodeling and Placental Senescence in a Pregnant Rat Model of Polycystic Ovary Syndrome

作者：Min Hu, Yuehui Zhang, Xu Zhang, XiuYing Zhang, Xinyue Huang, Yaxing Lu, Yijia Li, Mats Brännström,Amanda Nancy Sferruzzi-Perri, Linus . Shao, and Håkan Billigy

Cell Journal

Three-dimensional topography of eye-specific domains in the lateral geniculate nucleus of pigmented and albino rats after Tissue Clearing

作者：Huaixuan Sheng, , Mingru Huang, , Huizhu Li, , Luyi Sun, , Sijia Feng,, Xiner Du, Yicong Wang, Ph, Xiaoyu Tong, M.Sc., Yi Feng, M.D., Ph.D., Jun Chen, Ph.D. , Yunxia Li, M.D.

Cerebral Cortex

Three-dimensional topography of eye-specific domains in the lateral geniculate nucleus of pigmented and albino rats

作者：Hangqi Li, Qiuying Zhou, Yanlu Chen, Huijie Hu, Liang Gao, Toru Takahata

2022（6篇）

Cell Reports

Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection

作者：Qihang Hou, Junpeng Jia, Jian Lin, Linda Zhu, Shuang Xie, Qinghua Yu, Yuchen Li

Frontiers in Neurology

Knockdown of NRSF Alleviates Ischemic Brain Injury and Microvasculature Defects in Diabetic MCAO Mice

作者：Cheng-Feng He , Wen-Jiao Xue , Xiao-Die Xu , Jian-Tao Wang , Xin-Ru Wang ,Yi Feng, Hou-Guang Zhou and Jing-Chun Guo

Nature

Olfactory sensory experience regulates gliomagenesis via neuronal IGF1

作者：Pengxiang Chen, Wei Wang, Rui Liu, Jiahui Lyu,Lei Zhang, Baizhou Li, Biying Qiu, Anhao Tian, Wenhong Jiang, Honggang Ying, Rui Jing, Qianqian Wang, Keqing Zhu, Ruiliang Bai, Linghui Zeng, Shumin Duan& Chong Liu

Nature Communications

Carbonized paramagnetic complexes of Mn (II) as contrast agents for precise magnetic resonance imaging of sub-millimeter-sized orthotopic tumors

作者：Ruixue Qin, Shi Li, Yuwei Qiu, Yushuo Feng, Yaqing Liu, Dandan Ding, Lihua Xu, Xiaoqian Ma, Wenjing Sun& Hongmin Chen

Chinese Medicine

Three-dimensional visualization of electroacupuncture-induced activation of brown adipose tissue via sympathetic innervation in PCOS rats

作者：Hongru Gao, Xiaoyu Tong, Wei Hu, Yicong Wang, Kuinyu Lee, Xiaoqing Xu, Jiemei Shi, Zhenle Pei, Wenhan Lu, Yuning Chen, Ruonan Zhang, Zheyi Wang, Ziyu Wang, Chengzhi Han, Yu Wang and Yi Feng

Neuroscience Bulletin

Spatiotemporal Dynamics of the Molecular Expression Pattern and Intercellular Interactions in the Glial Scar Response to Spinal Cord Injury

Leilei Gong ,Yun Gu ,Xiaoxiao Han · Chengcheng Luan · Chang Liu · Xinghui Wang · Yufeng Sun · Mengru Zheng · Mengya Fang· Shuhai Yang · Lai Xu · Hualin Sun · Bin Yu · Xiaosong Gu · Songlin Zhou

2021（3篇）

Science

Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair

作者：Lingjuan He, Wenjuan Pu, Xiuxiu Liu, Zhenqian Zhang, Maoying Han, Yi Li, Xiuzhen Huang, Ximeng Han, Yan Li, Kuo Liu, Mengyang Shi, Liang Lai, Ruilin Sun, Qing-Dong Wang, Yong Ji, Jan S. Tchorz, Bin Zhou

Nanoscale

Concentrated small extracellular vesicles from menstrual blood-derived stromal cells improve intrauterine adhesion, a pre-clinical study in a rat model

作者：Siwen Zhang, Qiyuan Chang, Pingping Li, Xiaoyu Tong, Yi Feng, Xinyao Hao, Xudong Zhang, Zhengwei Yuane and Jichun Tan

STAR Protocols

Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues

作者：Ruili Feng, Dongyue Wang, Yanlu Chen, Jing Lu, and Liang Gao

2020（2篇）

Cell Reports 

A Versatile Tiling Light Sheet Microscope for Cleared Tissues Imaging

作者：Yanlu Chen, Xiaoliang, Dongdong Zhang, Chunhui Wang, Ruili Feng, Xuzhao Yao Wen,1,2,12 Hao Xu, Xinyi Shirley Zhang,6 Xiao Yang, Yongyi Chen, Yi Feng, Bo Zhou, Bi-Chang Chen,Kai Lei, Shang Cai, Jie-Min Jia and Liang Gao

中国光学期刊

光片荧光显微成像技术及应用进展

于湘华，刘超，柏晨，杨廷龙，彭彤，但且，闵俊伟，姚保利

C57整鼠

经yh533388银河组织透明化试剂盒处理，使用LS18平铺光片显微镜进行整体3D成像，可清晰观察小鼠全身组织分布和形态，

样本尺寸：37.3mm*77.4mm*23.1mm，

小鼠头部细节

小鼠胸部细节

Thy1-GFP成年小鼠完整中枢神经系统

采用yh533388银河亲水型透明化试剂盒制样，分辨率：2μm×2μm×5μm

Thy1-eGFP小鼠完整颅骨脑样本

采用yh533388银河透明化试剂盒，使用yh533388银河LS18平铺光片显微镜进行三维成像

神经细节展示

 小鼠全脑血管成像                                             小鼠肺成像                           小鼠肾脏血管成像   

     乳腺肿瘤成像                       小鼠骨双色成像                胎鼠卵巢双色成像              小鼠睾丸血管成像

   小鼠脾脏神经成像                   小鼠胚胎成像                     小鼠肠血管成像                   小鼠胃血管成像

更多精彩案例可在yh533388银河视频号观看：

技术参数：